BM1204 BIO CHEMISTRY AND HUMAN PHYSIOLOGY LAB 0 0 4 100
1. Recording of Muscle Response to Induced Electrical Stimulation
2. Study of rate of Conduction of Nerve Impulses.
3. Isolated Frog Heart Perfusion and Effect of ionic Changes.
4. Testing of Hearing using Tuning Fork.
5. Testing of various parameters of Vision and Errors of Refraction.
6. Testing for Detection of Glucose, Fructose and Starch.
7. General Test for Proteins
8. Testing of Urine for presence of Sugar, Protein
9. Estimation using Spectrophotometer
List of equipment for a batch of 30
Sl.No | Description | Quantity required |
1. | Recording of Muscle Response to induced Electrical Stimulation (i) Low voltage (D.C)unit (ii) DuBois Raymond induction coil with Neef’s hammer – to obtain alternate faradic current (iii) Vibrating Reed (iv) Stimulating electrodes (v) Tap key (vi) Tuning fork (100 Hz) | 1 No 2 Nos 2 Nos 5 Nos 5 Nos 2 Nos |
2. | Study of rate of Conduction of Nerve Impulses (i) Sherington – Starling drum (ii) Lucas trough / Muscle bath (iii) Writing lever with after load screw (Isotonic Muscle Lever) (iv) Electrical Kymograph (v) Muscle bath stand and S clamps Hook and weight | 2 Nos 2 Nos 4 Nos 2 Nos 2 Nos |
3. | Isolated Frog Heart perfusion and Effect of ionic changes (i) Mariotte’s bottle (Perfusion bottle) (ii) Rubber tubing (iii) Syme’s Cannula (iv) Frontal lever/ Starling’s Heart lever (v) Myograph board | 1 set |
4. | Testing of Hearing using Tuning Fork Tuning Fork with 256 Hz | 2 Nos |
5. | Testing of various parameters of Vision and Errors of Refraction Color Blindness – Isihara chart Visual acuity – Snellen’s chart Myopia + Hyperopia – by letters reading Ophthalmoscope – to view retina | 1set |
6. | Testing for Detection of Glucose, Fructose and Starch (i) Test for detection of Glucose Source – Blood Estimation – Spectrophotometer Reagent : Alkaline Cupric Tartarate Solution Phosphomolybdic acid (ii) Test for detection of Fructose Reagent : 1% alpha napthol Concentrated Hydrochloric acid (iii) Test for detection of starch Reagent : Iodine (iv) Test tube | 1 set |
7. | General Test for Proteins General test for Proteins Reagent : Concentrated Nitric acid | 1 set |
8. | Testing of Urine for Presence of Sugar, Protein Testing of Urine for Presence of Sugar, Protein of Sugar Reagent – Benedict’s reagent Protein Reagent – 1% Acetic acid | 1 set |
9. | Estimation using Spectrophotometer Spectrophotometer | 1 No |
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